1. Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora. Sasaki, N., Nishizaki, Y., Yamada, E., Tatsuzawa, F., Nakatsuka, T. Takahashi, H., Nishihara, M.  FEBS Letters 589: 182-187 [link]
  2. Achievements and perspectives in biochemistry concerning anthocyanin modification for blue flower coloration.                                Sasaki, N. and Nakayama, T. Plant and Cell Physiology 56:28-40 [ink]
  1. Molecular characterization of mutations in white-flowered torenia plants.                                                                                            Nishihara, M., Yamada, E., Saito, M., Fujita, K., Takahashi, H., Nakatsuka, T. BMC Plant Biology 14: 86. [Open Access]
  2. Silencing of DS2 aminoacylase-like genes confirms salicylic acid-dependent resistance to Phytophthora infestans in Nicotiana benthamiana. Nakano, M., Nishihara, M., Yoshioka, H., Ohnishi, K., Hikichi, Y., Kiba, A.   Plant Signaling & Behavior  9: e28004 (link) 
  3. 植物の香りと色の代謝工学が拓く新時代.  有村源一郎・西原昌宏・下田武志                                                                                        バイオサイエンスとインダストリー Vol.72 No.3 197-202. (link)
  4. High concentrations of sucrose induce overwintering bud formation in gentian plantlets cultured in vitro.                                             Imamura, T., Higuchi, A., Sekine, K.T., Yamashita, T., Takahashi, H. Plant Biotechnology 31: 97-104 [link]
  5. Mitsunami, T., Nishihara, M., Galis, I., Alamgir, KM., Hojo, Y., Fujita, K., Sasaki, N., Nemoto, K., Sawasaki, T., Arimura, G. (2014) Overexpression of the PAP1 transcription factor reveals a complex regulation of flavonoid and phenylpropanoid metabolism in Nicotiana tabacum plants attacked by Spodoptera litura. PLoS ONE 9: e108849. [PLoS One LINK]
  6. The gentio-oligosaccharide gentiobiose functions in the modulation of bud dormancy in the herbaceous perennial Gentiana.     Takahashi, H., Imamura, T., Konno, N., Takeda, T., Fujita, K., Konishi, T., Nishihara, M., Uchimiya, H.                                                Plant Cell  26:3949-3963  [link]
  7. Gentiolactone, a secoiridoid dilactone from Gentiana triflora, inhibits TNF-α, iNOS and Cox-2 mRNA expression and block NK-κB promoter activity in murine macrophages. Yamada, H., Kikuchi, S., Inui, T., Takahashi, H., and Kimura, K.I.                                           PLoS ONE. 9, e113834. [PLoS One LINK]
  8. Transcriptional regulators of flavonoid biosynthesis and their application to flower color modification in Japanese gentians.          Nakatsuka, T., Sasaki, N., Nishihara, M. Plant Biotechnology 31: 389-399 [link]
  1. Efficient haploid and double haploid production from unfertilized ovule culture of gentians (Gentiana spp.). Doi, H., Hoshi, N., Yamada, E., Yokoi, S., Nishihara, M., Hikage, T. and Takahata, Y.  Breeding Science 63: 400-406. [Open Access]
  2. Suppression of anthocyanin pigmentation by single MYB transcription factors from gentian. Nakatsuka, T., Yamada, E., Saito, M., Fujita, K. and Nishihara, M. Plant Cell Reports 32: 1925-1937. [Springer LINK]
  3. Suppression of DS1 phosphatidic acid phosphatase confirms resistance to Ralstonia solanacearum in Nicotiana benthamiana. Nakano, M., Nishihara, M., Yoshioka, H., Takahashi, H., Sawasaki, T., Ohnishi, K., Hikichi, Y. and Kiba, A. PLoS One 8: e75124.  [PLoS One LINK]
  4. Metabolic engineering of the C16 homoterpene TMTT in Lotus japonicus through overexpression of (E,E)-geranyllinalool synthase attracts generalist and specialist predators in different manners. Brillada,C.  Nishihara, M., Shimoda, T.,  Garms, S.,  Boland, W.,  Maffei, ME. and Arimura, G. New Phytologist 200: 1200-1211. [Wiley link]
  5. Frontiers of torenia research:  innovative ornamental traits and study of ecological interaction networks through genetic engineering.  Masahiro Nishihara, Takeshi Shimoda, Takashi Nakatsuka, Gen-ichiro Arimura. Plant Methods 9: 23. [Open Access]
  6. Genetic engineering of yellow betalain pigments beyond the species barrier.  Nakatsuka, T., Yamada, E., Takahashi, H., Imamura, T., Suzuki, M., Ozeki, Y., Tsujimura, I., Saito, M., Sakamoto, Y., Sasaki, N. and Nishihara, M. Scientific Reports 3: 1970. [Open Access]
  7. Inheritance of brown leaf spot disease resistance in gentians. Nekoduka, S., Horaguchi, H., Akasaka, S., Chiba, K., Hikage, T., Kawamura, H., Nakatsuka, T. and Nishihara, M. Journal of General Plant Pathology 79: 165-167. [Springer Link]
  8. Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome. Kobayashi, K., Atsumi, G., Iwadate, Y., Tomita, R., Chiba, K., Akasaka, S., Nishihara, M., Takahashi, H., Yamaoka, N., Nishiguchi, M. and Sekine, K. Journal of General Plant Pathology 79: 56-53 [Springer Link]
  9. Dehydrins are highly expressed in overwintering buds and enhance drought and freezing tolerance in Gentiana triflora. Imamura, T., Higuchi, A., and Takahashi, H. Plant Science 213: 55-66. [PubMed]
  10. Metabolite profiling reveals tissue- and temperature-specific metabolomic responses in thermoregulatory male florets ofDracunculus vulgaris (Araceae). Ito, K., Takahashi, H., Umekawa, Y., Imamura, T., Kawasaki, S., Ogata, T., Kakizaki, Y., and Seymour, R.S. Metabolomics 9: 919-930. [link]
  11. NAD+ Accumulation during Pollen Maturation in Arabidopsis Regulating Onset of Germination. Hashida, S.N., Takahashi, H., Takahara, K., Kawai-Yamada, M., Kitazaki, K., Shoji, K., Goto, F., Yoshihara, T., and Uchimiya, H. Molecular Plant 6: 216-25. [PubMed]

  1. Construction of the first genetic linkage map of Japanese gentian (Gentianaceae). Nakatsuka T, Yamada E, Saito M, Hikage T, Ushiku Y and Nishihara M. BMC Genomics 13: 672. [Open Access]
  2. Isolation and characterization of GtMYBP3 and GtMYBP4, orthologues of R2R3-MYB transcription factors that regulate early flavonoid biosynthesis, in gentian flowers. Nakatsuka T, Saito M, Yamada E, Fujita K, Kakizaki Y, Nishihara M. Journal of Experimental Botany 63: 6505-6517.  [PubMed]
  3. Acquired immunity of transgenic torenia plants overexpressing agmatine coumaroyltransferase to pathogens and herbivore pests. Muroi, A., Matsui, K., Shimoda, T., Kihara, H., Ozawa, R., Ishihara, A., Nishihara, M. and Arimura, G. Scientific Reports 2: 689. [Open Access]
  4. Isolation and characterization of an asparagine-rich protein that regulates hypersensitive cell death-mediated resistance in Nicotiana plants. Komori, D., Nishihara, M., Takahashi, A., Gupta, M., Yoshioka, H., Mizumoto, H., Ohnishi, K. and Hikichi, Y. and Kiba, A. Plant Biotechnology 29: 293-300.   
  5. Plant-plant-plant communications, mediated by (E)-β-ocimene emitted from transgenic tobacco plants, prime indirect defense responses of lima beans. Arimura, G., Muroi, A., Nishihara, M. Journal of Plant Interactions. 7: 193-196. [Abstract]
  6. The effect of genetically enriched (E)- β-ocimene and the role of floral scent in the attraction of the predatory mite Phytoseiulus persimilis to spider mite-induced volatile blends of torenia. Shimoda, T., Nishihara, M., Ozawa, R., Takabayashi, J., Arimura, G. New Phytologist 193: 1009-1021. [PubMed]
  7. Gentian lipid transfer protein homolog with antimicrobial properties confers resistance to Botrytis cinerea in transgenic tobacco. Kiba, A., Nakatsuka, T., Yamamura, S., Nishihara, M. Plant Biotechnology 29: 95-101.  [Abstract]
  8. Development of DNA markers that discriminate between white- and blue-flowers in Japanese gentian plants. Nakatsuka, T., Saito, M., Sato-Ushiku, Y., Yamada, E., Nakasato, T., Hoshi, N., Fujiwara, K., Hikage, T. and Nishihara, M. Euphytica 184: 335-344. [Springer Link]
  9. Modification of light quality improves growth and medicinal quality of clonal explants derived from herbal plant Gentiana. Takahashi, H., Yamada, H., Yoshida, C., and Imamura, T. Plant Biotechnology 29: 315-318. [link]
  10. Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes (shiitake mushroom). Konno, N., Takahashi, H., Nakajima, M., Takeda, T., and Sakamoto, Y. AMB Express 2: 29. [PubMed]
  11. Comparative metabolomics of developmental alterations caused by mineral deficiency during in vitro culture of Gentiana triflora. Takahashi, H., Imamura, T., Miyagi, A., and Uchimiya, H. Metabolomics 8: 154-163. [link]


  1. A single-base substitution suppresses flower color mutation caused by a novel miniature inverted-repeat transposable element in gentian. Nishihara, M., Hikage, T., Yamada, E. and Nakatsuka, T. Molecular Genetics and Genomics  286: 371-382.  [PubMed]
  2. The composite effect of transgenic plant volatiles for acquired immunity to herbivory caused by inter-plant communications. Muroi, A., Ramadan, A., Nishihara, M., Yamamoto, M., Ozawa R., Takabayashi, J. and Arimura, G. PLoS One 6(10): e24594.  [PLoS One LINK]
  3. Development of simple sequence repeat markers for identification of Japanese gentian cultivars. Sato-Ushiku, Y., Shimada, N., Saito, M., Yamada, E., Hikage, T., Nakatsuka, T. and Nishihara, M.  Journal of Japanese Society for Horticultural Science 80: 475-485.  [Abstract] 
  4. Gynogenesis in gentians (Gentiana triflora, G. scabra) : production of haploids and doubled haploid. Doi, H., Yokoi , S., Hikage, T., Nishihara , M., Tsutsumi, K. and Takahata, Y. Plant Cell Reports (in press). [PubMed]
  5. The gentian orthologues of FT/TFL1 gene family control floral initiation in Gentiana. Imamura, T., Nakatsuka, T., Higuchi, A., Nishihara, M. and Takahashi, H. Plant and Cell Physiology 52: 1031-1041. [PubMed]
  6. Production of picotee-type flowers in Japanese gentian by CRES-T. Nakatsuka, T., Saito, M., Yamada, E. and Nishihara, M. Plant Biotechnology 28: 173-180.  [J-STAGE]
  7. A flavonol synthase gene GtFLS defines anther-specific flavonol accumulation in gentian. Kimura, S., Nakatsuka, T., Yamada, E., Saito , M. and Nishihara, M. Plant Biotechnology 28: 211-221.  [J-STAGE]
  8. De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian. Yamasaki, S., Oda, M., Koizumi, N., Mitsukuri, K., Johkan, M., Nakatsuka, T., Nishihara, M. and Mishiba, K. Plant Biotechnology 28: .223-230. 2011. [J-STAGE]
  9. Epigenetic modifications of the 35S promoter in cultured gentian cells. Yamasaki, S. Oda, M., Daimon, H., Mitsukuri, K., Johkan, M., Nakatsuka, T., Nishihara, M. and Mishiba, K. Plant Science 180: 612-919. [PubMed]
  10. The new FioreDB database provides comprehensive information on plant transcription factors and phenotypes induced by CRES-T in ornamental and model plants. Mitsuda, N., Takiguchi, Y., Shikata, M., Sage-Ono, K., Ono, M., Sasaki, K., Yamaguchi, H., Narumi, T., Tanaka, T., Sugiyama, M., Yamamura, T., Terakawa, T., Gion, K., Suzuri, R., Tanaka, Y., Nakatsuka, T., Kimura, S., Nishihara, M., Sakai, T., Endo-Onodera, R., Saitoh, K., Isuzugawa, K., Oshima, Y., Koyama, T., Ikeda, M., Narukawa, M., Matsui, K., Nakata, M., Ohtsubo, N. and Ohme-Takagi, M. Plant Biotechnology 28: 123-130. [J-STAGE]
  11. Genetic engineering of flavonoid pigments to modify flower color in floricultural plants. Nishihara, M. and Nakatsuka, T.  Biotechnology Letters 33: 433-441. [PubMed]
  12. Fate of 13C in metabolic pathways and effects of high CO2 on the alteration of metabolites in Rumex obtusifoliusL. Miyagi, A., Takahara, K., Kasajima, I., Takahashi, H., Kawai-Yamada, M., and Uchimiya, H. Metabolomics 7: 524-535. [link]


  1. UDP-glucose:3-deoxyanthocyanidin 5-O-glucosyltransferase from Sinningia cardinalis. Nakatsuka, T.and Nishihara, M. Planta 232:383-392. 2010. [PubMed].
  2. Strict de novo methylation of the 35S enhancer sequence in gentian. Mishiba, K., Yamasaki, S., Nakatsuka, T., Abe, Y.,Daimon, H., Oda, M. and Nishihara, M. PLoS One 5(3): e9670. 2010. [PubMed]
  3. Two R2R3-MYB genes, homologues of petunia AN2, regulate anthocyanin biosyntheses in flower tepals, tepal spots and leaves of asiatic hybrid lily. Yamagishi, M., Shimoyamada, Y., Nakatsuka, T., Masuda, K. Plant and Cell Physiology: 51: 463-474. 2010. [PubMed]
  4. Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian. Nakatsuka, T., Mishiba, K., Kubota, A. Abe, Y., Yamamura, Y., Nakamura, N., Tanaka, Y. and Nishihara, M. Journal of Plant Physiol 167: 231-237. 2010. [PubMed]
  5. Genetic engineering of novel flower colors in floricultural plants: Recent advances via transgenic approaches. Nishihara, M. and Nakatsuka, T. Methods in Molecular Biology 589: Protocols for in vitro propagation of ornamental plants. S. Mohan Jain and Sergio J. Ochatt (eds). The Humana Press Inc. pp. 325-347. 2010. [PubMed]
  6. A novel factor FLOURY ENDOSPERM2 is involved in regulation of rice grain size and starch quality. She, K.C., Kusano, H., Koizumi, K., Yamakawa, H., Hakata, M., Imamura, T., Fukuda, M., Naito, N., Tsurumaki, Y., Yaeshima, M., Tsuge, T., Matsumoto, K., Kudoh, M., Itoh, E., Kikuchi, S., Kishimoto, N., Yazaki, J., Ando, T., Yano, M., Aoyama, T., Sasaki, T., Satoh, H., and Shimada, H. Plant Cell 22: 3280-3294. 2010. [PubMed
  7. Acquired resistance to the rice blast in transgenic rice accumulating the antimicrobial peptide thanatin. Imamura, T., Yasuda, M., Kusano, H., Nakashita, H., Ohno, Y., Kamakura, T., Taguchi, S., and Shimada, H. Transgenic Research 19: 15-424. 2010. [PubMed]
  8. Metabolome and photochemical analysis of rice plants overexpressing Arabidopsis NAD kinase gene. Takahara, K., Kasajima, I., Takahashi, H., Hashida, S.N., Itami, T., Onodera, H., Toki, S., Yanagisawa, S., Kawai-Yamada, M., and Uchimiya, H. Plant Physiology 152: 1863-1873. 2010. [PubMed
  9. Principal component and hierarchical clustering analysis of metabolites in destructive weeds; Polygonaceous plants. Miyagi, A., Takahashi, H., Takahara, K., Hirabayashi, T., Nishimura, Y., Tezuka, T., Kawai-Yamada, M., and Uchimiya, H. Metabolomics 6, 146-155. 2010.[Link]
  10. Nicotinate/nicotinamide mononucleotide adenyltransferase-mediated regulation of NAD biosynthesis protects guard cells from reactive oxygen species in ABA-mediated stomatal movement in Arabidopsis. Hashida, S.N., Itami, T., Takahashi, H., Takahara, K., Nagano, M., Kawai-Yamada, M., Shoji, K., Goto, F., Yoshihara, T., and Uchimiya, H. Journal of Experimental Botany 61: 3813-3825. 2010. [PubMed
  11. Characterization of a cellobiohydrolase (MoCel6A) produced by Magnaporthe oryzae. Takahashi, M., Takahashi, H., Nakano, Y., Konishi, T., Terauchi, R., and Takeda, T.  Appl. Environ. Microbiol. 76: 6583-6590. 2010. [PubMed
  12. Genetic relationships in the genus Gentiana based on chloroplast DNA sequence data and nuclear DNA content. Mishiba, K., Yamane, K., Nakatsuka, T., Nakano, Y., Yamamura, S., Abe, J., Kawamura, K., Takahata, Y. and Nishihara, M. Breeding Science 59: 119-127. 2009. [J-STAGE]
  13. Over-expression of Arabidopsis FT gene reduces juvenile phase and induces early fowering in ornamental gentian plants. Nakatsuka, T., Abe, Y., Kakizaki, Y., Kubota, A., Shimada, N. and Nishihara, M. Euphytica 168: 113-119. 2009. [SpringerLink]
  14. Metabolite profiling by capillary electrophoresis mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants. Takahashi, H., Munemura, I., Nakatsuka, T., Nishihara, M. and Uchimiya, H. 2009. The Journal of Horticultural Science and Biotechnology 84: 312-318. 2009. [LINK]
  15. Development of codominant DNA marker distinguishing pink from blue flowers in Gentiana scabra. Kakizaki, Y., Nakatsuka, T., Kawamura, H., Abe, J., Abe, Y., Yamamura S. and Nishihara, M. Breeding Research 11: 9-14. 2009. (in Japanese with English abstract)[J-STAGE]
  16. Identification of gentian cultivars using SCAR markers based on intron-length polymorphisms of flavonoid biosynthetic genes. Shimada, N., Nakatsuka, T., Nakano, Y., Kakizaki, Y., Abe, Y., Hikage, T. and Nishihara, M. Scientia Horticulturae 119: 292-296. 2009.[ScienceDirect]
  17. Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase2 gene. Takahashi, H., Takahara, K., Hashida, S.N., Fujimori, T., Kawai-Yamada, M., Yamaya, T., Yanagisawa, S., and Uchimiya, H. Plant Physiol. 151, 100-113. 2009. [PubMed
  18. Metabolite profiling by capillary electrophoresis mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants. Takahashi H, Munemura I, Nakatsuka T, Nishihara M, and Uchimiya H.J. Hortic. Sci. Biotechnol. 84, 113-119. 2009. [Link]
  19. Polyamines as a common source of hydrogen peroxide in host- and nonhost-hypersensitive response during pathogen infection. Yoda H, Fujimura K, Takahashi H, Munemura I, Uchimiya H, and Sano H.Plant Mol. Biol. 70, 103-112. 2009. [PubMed
  20. The role of NAD biosynthesis in plant development and stress responses. Hashida SN, Takahashi H, and Uchimiya H. Ann. Bot. 103, 819-824. 2009. [PubMed
  21. Functional genomics of the Dof transcription factor family genes in suspension-cultured cells of Arabidopsis thaliana. Tsujimoto-Inui Y, Naito Y, Sakurai N, Suzuki H, Sasaki R, Takahashi H, Ohtsuki N, Nakano T, Yanagisawa S, Shibata D, Uchimiya H, Shinshi H, and Suzuki K. Plant Biotechnol. 26: 15-18. 2009. [Link]
  22. Identification and characterization of R2R3-MYB and bHLH transcription factors regulating anthocyanin biosynthesis in gentian flowers. Nakatsuka, T., Haruta, K.S., Pitaksutheepong, C., Abe, Y., Kakizaki, Y., Yamamoto, K., Shimada, N., Yamamura, S. and Nishihara, M. Plant and Cell Physiology 49: 1818-1829. 2008. [PubMed]
  23. Cloning and characterization of the UDP-glucose:anthocyanin 5-O-glucosyltransferase gene from blue-flowered gentian. Nakatsuka, T., Sato, K., Takahashi, H., Yamamura, S., and Nishihara, M. Journal of Experimental Botany 59: 1241-1252. 2008. [PubMed]
  24. RIP and RALyase cleave the sarcin/ricin domain, a critical domain for ribosome function, during senescence of wheat coleoptiles. Sawasaki, T., Nishihara, M. and Endo, Y. Biochemical and Biophysical Research Communications 370: 561-565. 2008. [SpringerLink]
  25. Flower color modification of gentian plants by RNAi-mediated gene silencing. Nakatsuka, T., Mishiba, K., Abe, Y., Kubota, A., Kakizaki, Y., Yamamura, S. and Nishihara, M. Plant Biotechnology 25: 61-68. 2008. [J-STAGE]
  26. FioreDB: a database of phenotypic information induced by the chimeric repressor silencing technology (CRES-T) in Arabidopsis and floricultural plants. Mitsuda, N., Umemura, Y. Ikeda, M., Shikata, M., Koyama, T., Matsui,K., Narumi, T., Aida, R., Sasaki, K., Hiyama, T., Higuchi, T., Ono, M., Isuzugawa, K., Saitoh, K., Endo,R., Ikeda, K., Nakatsuka, T., Nishihara, M., Yamamura, S., Yamamura, T., Terakawa, T., Ohtsubo, N., and Ohme-Takagi, M. Plant Biotechnology 25: 37-44. 2008. [J-STAGE]
  27. Gentians: From gene cloning to molecular breeding.  Nishihara, M., Nakatsuka, T., Mizutani-Fukuchi, M., Tanaka, Y., Yamamura, S. Floricultural and Ornamental Biotechnology V, published by Global Science Books, UK. pp. 57-67. 2008.
  28. The cell death factor, cell wall elicitor of rice blast fungus (Magnaporthe grisea) causes metabolic alterations including GABA shunt in rice cultured cells. Takahashi H, Matsumura H, Kawai-Yamada M, and Uchimiya H. Plant Sig. Behav. 11: 945-953. 2008. [PubMed
  29. Difference in metabolite levels between photoatotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry. Takahashi H, Uchimiya H, and Hihara Y. J. Exp. Bot. 59: 3009-3018. 2008. [PubMed
  30. CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells. Negi J, Matsuda O, Nagasawa T, Oba Y, Takahashi H, Kawai-Yamada M, Uchimiya H, Hashimoto M, and Iba K. Nature 452: 483-486. 2008. [PubMed
  31. NMR based structure-activity relationship analysis of an antimicrobial peptide, thanatin, engineered by site-specific chemical modification: Activity improvement and spectrum alteration. Imamura T, Yamamoto N, Tamura A, Murabayashi S, Hashimoto S, Shimada H, and Taguchi S. Biochem Biophys Res Commun. 369: 609-615. 2008. [PubMed
  32. Rice exonuclease-1 homologue, OsEXO1, that interacts with DNA polymerase lambda and RPA subunit proteins, is involved in cell proliferation. Furukawa T, Imamura T, Kitamoto HK, and Shimada H. Plant Mol Biol. 66: 519-531. 2008. [PubMed]
  33. Induction of differential flower pigmentation patterns by RNAi using promoters with distinct tissue-specific activity. Nakatsuka, T., Pitaksutheepong, C., Yamamura, S. and Nishihara, M. Plant Biotechnology Reports 1: 251-257. 2007. [SpringerLink]
  34. Pathogenesis-related protein 1 homologue is an antifungal protein in Wasabia japonica leaves and confers resistance to Botrytis cinereain transgenic tobacco. Kiba, A., Nishihara, M., Nakatsuka , T. and Yamamura, S. Plant Biotechnology 24: 247-253. 2007. [J-STAGE]
  35. Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway.Suzuki, S., Nishihara, M,, Nakatsuka, T., Misawa, N., Ogiwara, I. and Yamamura, S. Plant Cell Reports 26: 951-959. 2007.  [PubMed]
  36. Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes. Nakatsuka, T., Abe, Y., Kakizaki, Y., Yamamura, S. and Nishihara, M.  Plant Cell Reports 26: 1951-1959. 2007. [PubMed]
  37. Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase (AtNMNAT) is required for pollen tube growth. Hashida SN, Takahashi H, Kawai-Yamada M, and Uchimiya H. Plant J. 49, 694-703. 2007. [PubMed
  38. A rice dihydrosphingosine C4 hydroxylase (DSH1) gene, which is abundantly expressed in the stigmas, vascular cells and apical meristem, may be involved in fertility. Imamura T, Kusano H, Kajigaya Y, Ichikawa M, and Shimada H. Plant Cell Physiol. 48: 1108-1120. 2007. [PubMed]
  39. Two different transposable elements inserted in flavonoid 3', 5'-hydroxylase gene contribute to pink flower coloration in Gentiana scabra. Nakatsuka, T., Nishihara, M., Mishiba, K., Hirano, H. and Yamamura, S. Molecular Genetics and Genomics 275: 231-241. 2006.[PubMed]
  40. Heterologous expression of two gentian cytochrome P450 genes can modulate the intensity of flower pigmentation in transgenic tobacco plants. Nakatsuka, T., Nishihara, M., Mishiba, K. and Yamamura, S. Molecular Breeding 17: 91-99. 2006. [SpringerLink]
  41. Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants. Nishihara, M., Nakatsuka, T., Hosokawa, K., Yokoi, T., Abe, Y., Mishiba, K. and Yamamura S. Plant Biotechnology 23: 25-32. 2006. [J-STAGE]
  42. Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes. Mishiba, K., Nishihara, M., Abe, Y., Nakatsuka, T., Kawamura, H., Kodama, K., Takesawa, T., Abe, J. and Yamamura, S. Plant Biotechnology 23: 33-382006. [J-STAGE]
  43. Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene usingAgrobacterium method. Sjahril, R., Chin, DP., Khan, RS., Yamamura, S., Nakamura, I., Amemiya, Y. and Mii, M. Plant Biotechnology 23: 191-194. 2006. [J-STAGE]
  44. Isolation and characterization of a cDNA encoding polyketide reductase in Lotus japonicus. Shimada, N., Nakatsuka, T., Nishihara, M., Yamamura, S., Ayabe, S. and Aoki, T. Plant Biotechnology 23: 509-513. 2006. [J-STAGE]
  45. Transgenic potatoes expressing wasabi defensin peptide confers partial resistanceto gray mold (Botrytis cinerea). Khan, RS., Nishihara, M., Yamamura, S., Nakamura, I. and Mii, M. Plant Biotechnology 23: 179-183. 2006. [J-STAGE]
  46. Bisexual sterility conferred by the differential expression of Barnase and Barstar: a simple and efficient method of transgene containment. Kobayashi, K., Munemura, I., Hinata, K. and Yamamura, S. Plant Cell Reorts 25: 1347-1354. 2006. [PubMed]
  47. Chloroplast NAD kinase is essential for energy transduction through the xanthophyll cycle in photosynthesis. Takahashi H, Watanabe A, Tanaka A, Hashida SN, Kawai-Yamada M, Sonoike K, and Uchimiya H. Plant Cell Physiol. 47: 1678-1682. 2006. [PubMed
  48. Evaluation of metabolic alteration in transgenic rice overexpressing dihydroflavonol-4-reductase. Takahashi H, Hayashi M, Goto F, Sato S, Soga T, Nishioka T, Tomita M, Kawai-Yamada M, and Uchimiya H. Ann. Bot. 98: 819-825. 2006. [PubMed
  49. Polyamine oxidase is one of the key elements for oxidative burst to induce programmed cell death in tobacco cultured cells. Yoda H, Hiroi Y, Sano H. Plant Physiol. 142: 193-206. 2006. [PubMed]
  50. Temporal expression of flavonoid biosynthesis-related genes regulates flower pigmentation in gentian plants. Nakatsuka, T., Nishihara, M., Mishiba, K. and Yamamura, S. Plant Science 168: 1309-1318. 2005 [ScienceDirect].
  51. Two different mutations are involved in the formation of white-flowered gentian plants. Nakatsuka,T., Nishihara, M., Mishiba, K. and Yamamura, S. Plant Science 169: 949-958. 2005. [ScienceDirect]
  52. Consistent transcriptional silencing of 35S-driven transgenes in gentian. Mishiba, K., Nishihara, M., Nakatsuka, T., Abe, Y., Hirano, H., Yokoi, T., Kikuchi, A. and Yamamura, S. The Plant Journal 44: 541-556. 2005. [PubMed]
  53. Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. Nishihara, M., Nakatsuka, T. and Yamamura, S. FEBS Letters 579: 6074-6078. 2005. [PubMed]
  54. A peroxiredoxin Q homolog from gentians is involved in both resistance against fungal disease and oxidative stress. Kiba, H., Nishihara, M., Tsukatani, N., Nakatsuka, T., Kato, Y. and Yamamura, S. Plant and Cell Physiology 46: 1007-1015. 2005. [PubMed]
  55. Comparison of selection efficiency between the bar and hpt genes in Agrobacterium-mediated transformation of Muscari armeniacum. Suzuki, S., Nakano, M., Koike, Y., Ueda, K., Inoue, M., Nishihara, M. and Yamamura, S. Journal of the Japanese Society for Horticultura Science 74: 60-62. 2005. [PDF]
  56. Tanaka N, Takahashi H, Kitano H, Matsuoka M, Akao S, Uchimiya H, and Komatsu S. (2005). Proteome approach to characterize the methylmalonate-semialdehyde dehydrogenase that is regulated by gibberellin. J. Proteome Res. 4, 1575-1582. [PubMed
  57. Hayashi M, Takahashi H, Kawai-Yamada M, Tezuka T, and Uchimiya H. (2005). Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice. Proc. Natl. Acad. Sci. U. S. A. 102, 7020-7025. [PubMed]
  58. Transgenic rice expressing wasabi defensin gene exhibit its enhanced resistance to blast fungus (Magnaporthe grisea). Kanzaki, H., Nirasawa, S., Saito, H., Ito, M., Nishihara, M., Yamamura, S., Suzuki, K., Terauchi, R. and Nakamura I. In '' Rice Blast:Interaction with Rice and Control. " by eds. Shinji Kawasaki. Kluwer Academic. 2004.
  59. cDNAs for the synthesis of cyclic carotenoids in petals of Gentiana lutea and their transcriptional regulation during flower development. Zhu C. F., Yamamura, S., Nishihara, M., Koiwa, H. and Sandmann G. Biochimica et Biophysica Acta 1625: 305-308. 2003. [PubMed]
  60. C-terminal domain of a hevein-like protein from Wasabia japonica has potent antimicrobial activity. Kiba, A., Saitoh, H., Nishihara, M., Omiya, K. and Yamamura, S. Plant and Cell Physiology 44: 296-303. 2003. [PubMed]
  61. Control of in vitro organogenesis by cyclin-dependent kinase activities in plants. Yamaguchi, M., Kato, H., Yoshida, S., Yamamura, S., Uchimiya, H. and Umeda, M. Proceedings of the National Academy of Sciences of the United States of America 100: 8019-8023. 2003.[PubMed]
  62. Over-expression of Arabidopsis CAP causes decreased cell expansion leading to organ size reduction in transgenic tobacco plants. Barrero, R.A., Umeda, M., Yamamura, S. and Uchimiya, H. Annals of Botany 91: 599-603. 2003. [PubMed]
  63. cDNA cloning and expression of carotenogenic genes during flower development in Gentiana lutea. Zhu, C.F., Yamamura, S., Koiwa, H., Nishihara, M. and Sandmann, G. Plant Molecular Biology 48:277-285. 2002. [PubMed]
  64. Arabidopsis CAP Regulates the Actin Cytoskeleton Necessary for Plant Cell Elongation and Division. Barrero, R.A., Umeda, M., Yamamura, S. and Uchimiya, H. The Plant Cell 14: 149-163. 2002.  [PubMed]
  65. Production of antimicrobial defension in Nicotiana benthamiana with a potato virus vector. Saitoh, H., Kiba, A., Nishihara, M., Yamamura, S., Suzuki, K. and Terauchi, R. Molecular Plant-Microbe Interaction 14: 111-115. 2001. [PubMed]